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KMID : 0986520120120020087
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Korean Journal of Endoscrine Surgery
2012 Volume.12 No. 2 p.87 ~ p.91
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Detection of BRAFV600E Mutation using Peptide Nucleic Acid-Mediated PCR Clamping Method in Fine Needle Aspiration Cytology
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Hong Jong-Chul
Park Heon-Soo
Kang Myung-Koo
Lee Joon-Hyung
Kim Deok-Kyu
Park Mi-Kyoung
Kang Do-Young
Kim Sung-Heun
Hong Sook-Hee
Kim Dae-Cheol
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Abstract
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Purpose: The aim of this study was to evaluate the diagnostic value of a peptide nucleic acid (PNA)-mediated PCR clamping method for the detection of BRAFV600E mutations in fine needle aspiration cytology (FNAC).
Methods: One hundred sixty four patients underwent FNAC to evaluate BRAFV600E mutations between April 2011 and November 2011. Among them, forty-two patients were diagnosed with papillary thyroid carcinoma in a permanent pathologic specimen. A PNA-mediated PCR clamping method and a Dual-Priming Oligonucleotide (DPO)-based Real-time PCR method were used to detect the BRAFV600E mutation. We compared the result of mutation between the two methods.
Results: A BRAF mutation was found in 31 samples created by the PNA-mediated PCR clamping method, and in 28 samples in the DPO-based Real-time PCR method. The rate of BRAF mutation was 73.8% in association with the PNA-mediated PCR clamping method, and 66.7% in association with the DPO-based Real-time PCR method. There was no statistical differences between the two methods (P＞0.05).
Conclusion: The PNA-mediated PCR clamping method may be an alternative to the DPO-based Real-Time PCR method for detection of BRAF mutations in thyroid nodules.
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KEYWORD
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Thyroid cancer, BRAF mutation
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